The invention relates to a vector for cloning an insert having blunt ends, enabling recombinant clones to be positively selected, to a method for cloning an insert in said vector, and to the use of said cloning vector especially for expressing an insert.
According to the method of the present invention, the PCR product cloning vector designed to be advantageous for blue/white cloning selection can be produced with improved efficiency compared with the convention method.
This aspect of the invention is accomplished through recombination between: (a) a large-capacity cloning vector carrying a viral genome, and (b) a transfer vector containing the transgene of interest.
A group of cloning vector plasmids for use in constructing DNA molecules, such as transgenes, for the purpose of gene expression or analysis of gene expression.
Such modifications include generating insertions, deletions, substitutions, and/or point mutations at any chosen site in the independent origin based cloning vector.
The invention also concerns a polynucleotide coding for such a fusion polypeptide, a cloning and/or expression vector containing such a polypeptide as well as a recombinant cellular host containing such a vector.
Also, a library of rearranged immunoglobulin genes in a cloning vector is introduced into host cells.
For that purpose the merR regulatory gene was isolated from the pMOL30 plasmid of Cupriavidus metallidurans, line CH34 (SEQ ID NO. 1) and inserted into the pGEMT cloning vector, producing the PGEMT-Hg plasmid (SEQ ID NO. 2).