The cells used are selected on the basis of growth ability in culture and stability of the spontaneous mutation frequency. Tests conducted in vitro generally require the use of an exogenous source of metabolic activation.
The minimal number of viable cells surviving treatment and used at each stage in the test should be based on the spontaneous mutation frequency.
It is said that the Ribera del Xúquer region is the cradle of the Rojo Brillante persimmon, as that is where this variety emerged as a result of a spontaneous mutation.
Methods of reducing spontaneous mutation rate of a cell in a subject in need thereof by reducing endogenous levels of miR-155 are described.
The subject invention relates in part to the discovery of a spontaneous sunflower mutation.
Cell types used in this test should have a demonstrated sensitivity to chemical mutagens, a high cloning efficiency and a stable spontaneous mutant frequency.