Expression of the nucleic acids results in a multigenic phenotype in the tranfected cell.
Conjoint polynucleotide segments that contribute to or disrupt elements of a multigenic phenotype are produced and expressed in cells of interest.
The new expression cassettes are also useful in the ultimate production of a multigenic system for a one-step conversion of cholesterol into hydrocortisone.
The host cells are also useful for the ultimate preparation of a multigenic system for the one-step conversion of cholesterol into hydrocortisone.
In particular, the invention relates to a method for identifying a HLA-B*0702-restricted peptide which can trigger a cytotoxic response against several antigens from one single multigenic family, and to several such epitopes.
A carotenogenic biosynthetic gene cluster has been isolated from Panteoa stewartii strain DC413, wherein the genetic organization of the cluster is crtE-idi-crtX-crtY-crtI-crtB-crtZ.
The daptomycin biosynthetic gene cluster may be derived from Streptomyces, preferably from S. roseosporus.
Subsequently, mRNA is subjected to multigene analysis such as DNA arrays.
The invention provides nucleic acid molecules comprising all or a part of a daptomycin biosynthetic gene cluster.
The gene cluster, genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids.
Further disclosed is a multigene locus encoding all nine homologous 28-kDa protein genes of Ehrlichia canis.
Further, a multigene family of ribosome-inactivating-protein encoding genes of Trichosanthes Kirilowii is also described.
In particular, the present invention provides the ansamitocin biosynthetic gene cluster for the production of novel maytansinoid analogs with potent antitumor activity and reduced human toxicity.
The invention relates to the field of enzymatic gene amplification, and particularly to the provision of multigene expression profiles derived from messenger RNA.
The present invention relates to the identification of haplospecific geometric elements (HGEs) in a multigene cluster comprising genes encoding complement control proteins.
The 28-kDa outer membrane proteins (P28) of Ehrlichia chaffeensis are encoded by a multigene family consisting of 21 members located in a 23-kb DNA fragment in the genome of E. chaffeensis.
The present invention is directed to the cloning, sequencing and expression of homologous immunoreactive 28-kDa protein genes, p28-1, -2, -3, -5, -6, -7, -9, from a polymorphic multiple gene family of Ehrlichia canis.
Three of the clones, E8, E80, and E46, contain open reading frames for four highly homologous proteins which appear to be part of a multigene family resembling the MSP-2 gene family of Anaplasma marginale.
In addition, the present invention is directed to methods for generating multigene expression cassettes, methods for producing multiprotein complexes in vitro and in vivo, and methods for producing a vaccine.