An optimum reaction medium for performing nucleic acid detection and a method employing said medium are described.
The reaction medium is mixed in a reactor through circulation of the reaction medium back to the reactor.
The obtained reaction medium is neutralized, thereby forming by-products mainly consisting of salts, which are removed from the reaction medium.
The reactor system includes a heat exchanger for heating the reaction medium and a disengagement vessel for disengaging vapor from the heated reaction medium.
Preferred water soluble silica sources comprise silicic acid, or po!ysilicic acids, The aqueous reaction, medium is prepared by combining one or more nonionic surfactants and water, theteby forming an aqueous, reaction medium. comprising micelles.
Sodium silicate promoting MQ resin can be incorporated in the reaction medium.
The elemental sulfur is recovered by separating it from the reaction medium (1).
The encapsulated catalyst may recovered from the reaction medium and re-cycled.
The invention further concerns a reaction medium containing such a substrate or such a combination of substrates.