Several clones containing lactoferrin gene fragments were isolated from a mouse (129/J) genomic library including clone μJ14 which contains 7.5 kbp 5'-flanking sequence.
The invention relates to a novel protein, identified by screening a Anisakis simplex cDNA library, and to the encoding sequence and expression vectors thereof.
The invention provides a method of library construction suitable for gene regions of a higher flowering plant containing a complex genome.
The gene encoding the M. leprae 65kD antigen was sequenced and a lambda gt11 gene sublibrary was constructed with fragments of the gene.
The DNA shuffling technique is used to create a library of shuffled genes, and this is expressed in a suitable expression system and the expressed proteins are screened for lipolytic enzyme activity.
The present invention provides a polynucleotide (The upper and lower cases are to be respected as per the original abstract) isolated from a breast tumor library which identifies and encodes a human cachexia-associated protein (HCAP).
In the method of the invention, the gene regions of the genome are enriched by a methylation-sensitive enzyme instead of a conventional non-methylation-sensitive enzyme, and then a library is constructed.
The novel Gb1c cDNA encodes a protein of 899 amino acids differering from the previously noted gb1c sequence (Genbank Accession No. AJ012187) by a single amino acid substitution (a glutamine in place of a histidine at position 21).