Staining with the fluorochromes is effected with the use of intercalater type fluorochrome(s), mirror group type fluorochrome(s), and fluorochrome(s) covalently bonded to the nucleic acid.
The detection conjugate has a fluorochrome portion bound to the antibody portion and a linker bound to the fluorochrome portion, wherein the linker comprises an oligonucleotide.
The approach also provides for more accurate quantitative data regarding fluorochrome emissions amplitudes.
Said compounds may be used in any method utilizing a fluorochrome, a chromogen, or both.
A preoperative filling of the Schlemm's canal with a biocompatible fluorochrome, which can be excited using a pilot laser (7), serves to prevent a complete perforation.
The probe comprises a detectable label, linked to its 5' end as well as a quencher, linked to its 3' end.
In preferred embodiments, the substrate is labelled with a fluorescent dye selected from the acridone and the quinacridone classes of dyes.
The invention provides methods for visualizing hepatobiliary structures, or lesions in a liver, intraoperatively by use of fluorescent dyes.
The proteins are separately labeled with fluorochromes which, when in close spatial proximity, preferably, less than about 6 nm, interact through the transfer of energy from one fluorochrome to the other.
The method can be performed with solid supports of a single average size and a single fluorochrome and without the need for using other detection systems (fluorescence FS & SS).
Disclosed are methods for accurately determining the total emission intensity of a single fluorochrome, under imaging conditions, using a digital imaging fluorescence microscopy system.