Switch of the agitator and the aerator and allow the activated sludge to settle.
The aeration is then stopped, the sludge allowed to settle and the supernatant liquor is removed.
Gently tap the loaded column at its lower end to settle the aluminium oxide.
Thereafter, undissolved components are allowed to settle and the dissolved oil is separated from the supercritical fluid.
At the end of the period the agitation ceases, and the bed of adsorbent material is allowed to settle.
Decant the liquid through the crucible.
Allow to cool and decant off the acid.
Centrifuge for two minutes and decant the ethyl acetate phase through a glass fibre filter (4.6), into a 500 ml separating funnel.
Decant the supernatant liquid through the weighed filter crucible.
Decant the liquid through the weighed filter crucible.
Extraction using a separating funnel (4.2.3
Separation funnel or conical bottomed settling beaker.
Separation funnel pieces shall be disassembled before cleaning.
Combine the three ether extracts in one separating funnel containing 50 ml of water.
Shake the separating funnel vigorously for two minutes and allow the phases to separate.
Run the lower acid layer into a 250 ml separating funnel.
Allow the layers to separate and transfer the upper layer to the separating funnel as before.
Separation funnels and glassware need washing in a washing machine.
The digestion fluid is allowed to stand in the funnel for 30 minutes.
The resulting mixture is allowed to separate into two layers, a first layer and a second layer, The first layer, including the food grade solvent, flavors, color, alcohol (i.e., ethanol) and water, is separated from the second layer.
This invention incorporates barrel-shaped polymer-structured separatory funnels.
Separation funnel pieces and glassware shall be pre-washed manually and then washed in a washing machine.