The compositions and methods utilize collagenase, e.g., bacterial collagenase, other isolated collagenase, or synthetic collagenase, e.g., recombinant collagenase.
A drug product comprising a combination of highly purified collagenase I and collagenase II from Colostridium histolyticum is disclosed.
A process for purifying crude collagenase is disclosed.
The field of the invention relates to percutaneous interventions of occluded arteries using local infusion of collagenase or a combination of enzymes that include collagenase.
The drug product includes collagenase I and collagenase II in a ratio of about 1 to 1, with a purity of greater than at least 95%.
The specific enzymes are collegenase(IV) and elastase.
The compound (I) and the salt maintain a potent collagenase inhibitor activity in the blood for long after being administered, and are useful as a remedy for various diseases believed to be caused by an increased collagenase activity.
In the enzymatical treatment of the fat tissue, of the decidual or amniotic placenta membrane collagenase of the type I is used, and in the enzymatical treatment of the chorion placenta stroma collagenase of the type IV is used.
By purifying the thus-obtained fusion collagenase by affinity chromatography, a collagenase having a collagen-binding site can be selectively collected.
The invention encompasses methods wherein the dose of collagenase per injection is between about 50 to about 200 ABC units and/or wherein the concentration of collagenase is between about 50 to about 2000 ABC units/milliliter (ml).
Also provided is a drug product which includes collagenase I and collagenase II at an optimized fixed mass ratio, and which has a purity of greater than at least 95%.
The invention relates to recombinant nucleic acid and polypeptides encoding collagenase I and collagenase II, methods for the preparation thereof and methods for the use thereof.
Preferably the substrate comprises a substrate for elastase or a collagenase.
Transglutaminase treatment is also shown to improve resistance to abrasion and collagenase.
An isolated sequence is provided that encodes crab serine collagenase I. The mature recombinant collagenase (SEQ.