The methods of the subject invention are capable of providing high percentages of totipotent cells.
Such blastomere-like totipotent cells can be differentiated into ectodermal, mesodermal, or endodermal tissues, including placental tissues and germ cells.
The invention relates in part to totipotent cells useful for cloning porcine animals, porcine embryos produced form such cells by employing nuclear transfer techniques, and porcine animals that arise from such cells and embryos. .
These diploid NT units e.g., human NT units, may be used to obtain pluripotent cells and differentiated cells and tissues.
The methods of the invention find application in the preparation of totipotent or pluripotent cells of substantially identical genotype, as well as the cloning of vertebrates.
A large-scale sequencing project has identified important markers that are characteristic of undifferentiated pluripotent cells.
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