In some embodiments, the messenger RNA also comprises a polyA tail.
In some embodiments, the messenger RNA also comprises an m7GpppG cap.
In some embodiments, the purified preparation of messenger RNA is significantly less immunogenic than an unpurified preparation of messenger RNA with the same sequence.
The hybrid genes respond to sterols by decreasing the production of messenger RNA.
The messenger RNA stabilising elements are preferably from the hrpA gene.
Methods for affecting mRNA expression or translation through the modification of pre-mRNA or mRNA transcripts are described.
Then use is made of two primers between which the channel protein mRNA or a part of the mRNA region is located by using the thus extracted mRNA as a template.
Degradation of mRNA transcribed from recombinant DNA is decreased by decreasing the length of the untranslated region of the mRNA.
Such methods involve the incorporation of biotinylated rNTP analogues into cellular mRNA, and separating biotinylated mRNA from unlabeled RNA.
Cleavage of the mRNA results in an altered cell phenotype from which the function of the product encoded by the mRNA is determined.
The mRNA that can be detected has a unique sequence.
Methods are provided for the isolation of newly synthesized mRNA.
When it is translated from an edited RNA messenger, it is secreted.
The gene targeting message RNA may in turn be the product of transcription of a gene targeting construct (GTC) encoding the gene targeting message RNA.
Furthermore, the present invention relates to antisense oligonucleotide derivatives directed against both human bcl-xL nRNA and human bcl-2 mRNA, and being capable of modulating the biosynthesis of both human bcl-xl protein and human bcl-2 protein.
The invention also relates to a product and a kit containing the mRNA and cytokine or cytokine mRNA, or CpG DNA or adjuvant RNA according to the invention.
A method for increasing the translation efficiency of a mRNA sequence is provided.
The present invention provides a method for detecting and quantifying mRNA in a sample.
The first polynucleotide has a first sequence that hybridizes to the unique sequence on the mRNA.
The pattern of expression of SCL mRNA is primarily predominant in early hematopoietic tissues.
These levels are then used to estimate corresponding mRNA levels in liver.
A process of improving the quality of the mRNA that will be used for genetic analysis.
These 'antisense' oligonucleotides are hybridizable to the c-kit mRNA transcript.