Provided is a single-stranded nucleic acid which can inhibit the expression of a target gene having a delivering function.
The detection agent contains a linear first single-stranded nucleic acid attached to a capture moiety that specific binds the target molecule of interest.
The invention includes biosensors systems in which fluorescent molecules are tethered to immobilized single-stranded nucleic acid.
By amplifying a double-stranded DNA having the above-described restriction enzyme recognition site with the use of an LAMP reaction, a single-stranded nucleic acid can be more efficiently synthesized.
The second single stranded nucleic acid portion (2) is located between the first (1) and the third (3) single stranded nucleic acid portions.
The first (1) and the third (3) single stranded nucleic acid portions build a double stranded, intramolecular stem (10).
The method is applicable for use with either double stranded or single stranded nucleic acid, and can be used to bind oligonucleotides and/or polymerase chain reaction products.
The second single stranded nucleic acid portion (2) forms a single stranded oligonucleotide loop (20) with a nucleotide sequence that is complementary to individual SNP nucleic acid target sequences.
The first (1) and the third (3) single stranded nucleic acid portions of the stem-loop probe comprise a 3' or 5' end configured as an A, T, or C nucleotide, to which A, T, or C nucleotide a non-quenched fluorophore is conjugated.